New Strategy for Precise Genome Editing Developed by ECUST Team, Published in Nucleic Acids Research

Recently, the research team led by Associate Professor Shengmin Zhou from the State Key Laboratory of Bioreactor Engineering and the School of Biotechnology at ECUST, has introduced a highly efficient and universal strategy for precise genome editing. Their findings offer a scalable solution to a long-standing challenge in the field: efficiently knocking in target DNA fragments without relying heavily on specific insertion sites.

The study, titled “Dual-single-guide RNA strategy improves CRISPR-mediated homology-directed repair in Aspergillus”, was recently published in Nucleic Acids Research. By systematically elucidating the DNA repair mechanisms that typically limit CRISPR-Mediated knock-in efficiency, the team successfully developed a predictable and broadly applicable homology-directed repair (HDR) design strategy.

The first author of the article is Fu Mingxin, a master’s student from the School of Biotechnology at ECUST. The corresponding author is Associate Professor Shengmin Zhou from the State Key Laboratory of Bioreactor Engineering.

This work was supported by the National Natural Science Foundation of China and other funding programs.